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Image Search Results
Journal: Life Science Alliance
Article Title: p53 ensures the normal behavior and modification of G1/S-specific histone H3.1 in the nucleus
doi: 10.26508/lsa.202402835
Figure Lengend Snippet: (A) Representative immunoblots using the indicated antibodies. Normalized lipin 1/β-tubulin ratios are shown. n = 3 biological replicates; ** P < 0.01, * P < 0.05, and NS, not significant, one-way ANOVA followed by Dunnett’s multiple comparisons test. (B, C) Quantification of NEP1R1 (B) and TMEM255A (C) mRNA (normalized by ACTB mRNA) in H1299 cells. n ≥ 3 biological replicates; ** P < 0.01, * P < 0.03, and NS, not significant, two-tailed unpaired t test. (D) Quantification of TMEM255A mRNA (normalized by ACTB mRNA). n ≥ 3 biological replicates; * P < 0.02 and NS, not significant, two-tailed unpaired t test. (E) Representative immunoblots using the indicated antibodies. IP, immunoprecipitation. (F) Representative immunoblots using the indicated antibodies. V, vehicle. Normalized eCTDNEP1/β-tubulin ratios are shown. n = 3 biological replicates; ** P < 0.01, one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) Representative immunoblots using the indicated antibodies. (H) Representative immunoblots using the indicated antibodies. IP, immunoprecipitation. WB, Western blotting. The arrowhead indicates endogenous CTDNEP1, and the arrow indicates eCTDNEP1. Normalized CTDNEP1/β-tubulin ratios of the DMSO-treated samples are shown at the bottom. n = 3 biological replicates; P = 0.053, two-tailed unpaired t test. (I) Model for the inhibition of the perinuclear accumulation of H3K27me3 regulated by p53. Source data are available for this figure.
Article Snippet: Retroviruses with the vesicular stomatitis virus G (VSV-G) envelope were produced by transfection of GP2-293 cells (Clontech) with the pRetroX construct and pVSV-G (Clontech) using Lipofectamine LTX reagent (Invitrogen), following the manufacturer’s instructions. cDNAs encoding CTDNEP1 ,
Techniques: Western Blot, Two Tailed Test, Immunoprecipitation, Inhibition